Nansen Legacy Bacterial and Archaeal community composition

Stefan Thiele

doi:10.21335/nmdc-831634754

Nansen Legacy Bacterial and Archaeal community composition

Citation

Thiele S (2023). Nansen Legacy Bacterial and Archaeal community composition. Version 1.3. The Nansen Legacy Project. Occurrence dataset https://doi.org/10.21335/nmdc-831634754 accessed via GBIF.org on 2024-07-11.

Description

The bacterial and archaeal community composition based on 16S rRNA metabarcoding of seawater samples taken along the transect of the Nansen Legacy project (https://www.wikidata.org/wiki/Q110857128) from 2018 to 2021. The samples contain 7 datasets from different cruises at different time points. From the samples the DNA was extracted and then used for 16S rRNA gene sequencing using the standard Earth Microbiome Project primers (515F and 806R) and Illumina MiSeq paired end sequencing. The raw sequences were run using the DADA2 pipeline (Callahan et al., 2016) and then cleaned for samples with under a total of 10000 sequences and ASVs with less than a total of 3 sequences per sequencing run or 0.0001% relative abundance.

Sampling Description

Study Extent

The samples were taken on 7 cruises along the Nansen Legacy Project transect in the Northern Barents Sea.

Sampling

Please refer to the Nansen Legacy Sampling Protocol: https://arvenetternansen.com/sampling-protocol-collection/

Method steps

Please refer to the Nansen Legacy Sampling Protocol: https://arvenetternansen.com/sampling-protocol-collection/

Taxonomic Coverages

Bacteria and Archaea based on 16S rRNA gene sequence.

Bacteria and Archaea
rank: domain

Geographic Coverages

Northern Barents Sea, east of Svalbard, Norway.

Bibliographic Citations

Contacts

Stefan Thiele
originator
position: Postdoc
University of Bergen
Thormohlensgate 53 A/B
Bergen
5006
Vestland
NO
email: stefan.thiele@uib.no
userId: http://orcid.org/0000-0002-4322-3795

Stefan Thiele
metadata author
position: Postdoc
University of Bergen
Thormohlensgate 53 A/B
Bergen
5006
Vestland
NO
email: stefan.thiele@uib.no
userId: http://orcid.org/0000-0002-4322-3795

Stefan Thiele
user
position: Postdoc
University of Bergen
Thormohlensgate 53 A/B
Bergen
5006
Vestland
NO
email: stefan.thiele@uib.no
userId: https://orcid.org/0000-0002-4322-3795

Stefan Thiele
administrative point of contact
position: Postdoc
University of Bergen
Thormohlensgate 53 A/B
Bergen
5006
Vestland
NO
email: stefan.thiele@uib.no
userId: http://orcid.org/0000-0002-4322-3795